ARG82545

Human Semaphorin 6D ELISA Kit

Human Semaphorin 6D ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82545-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82545-002 Standard 2 X 10 ng/vial 4°C
ARG82545-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82545-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82545-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82545-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82545-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82545-008 25X Wash buffer 20 ml 4°C
ARG82545-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82545-010 STOP solution 10 ml (Ready to use) 4°C
ARG82545-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82545 Human Semaphorin 6D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Semaphorin 6D in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Semaphorin 6D
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 20 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.7%
Alternate Names Semaphorin-6D

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80031 Human SEMA6D

Swiss-port # Q8NFY4 Human Semaphorin-6D

Gene Symbol SEMA6D
Gene Full Name sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Background Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010]
Function Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Cell membrane; Single-pass type I membrane protein. Isoform 4: Cell membrane; Single-pass type I membrane protein. Isoform 5: Cell membrane; Single-pass type I membrane protein. Isoform 7: Cytoplasm. [UniProt]
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