ARG81604

Human Serum amyloid P-component ELISA Kit

Human Serum amyloid P-component ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81604-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81604-002 Standard 2 X 100 ng/vial 4°C
ARG81604-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81604-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81604-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81604-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81604-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81604-008 25X Wash buffer 20 ml 4°C
ARG81604-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81604-010 STOP solution 10 ml (Ready to use) 4°C
ARG81604-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81604 Human Serum amyloid P-component ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Serum amyloid P-component in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Serum amyloid P component
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.78 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 1.56 - 100 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 6.1%
Alternate Names 9.5S alpha-1-glycoprotein; HEL-S-92n; PTX2; Serum amyloid P-component; 1-203; SAP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 325 Human APCS

Swiss-port # P02743 Human Serum amyloid P-component

Gene Symbol APCS
Gene Full Name amyloid P component, serum
Background The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]
Function Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. [UniProt]
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PTM N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). [UniProt]
Title Download Link
ARG81604 Human Serum amyloid P-component ELISA Kit user's manual download icon Download