ARG70342

Human Siglec 15 recombinant protein (Active) (ECD) (His-tagged, C-ter)

Human Siglec 15 recombinant protein (Active) (ECD) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Active Human Siglec 15 recombinant protein (ECD).
Tested Reactivity Hu
Tested Application FuncSt, SDS-PAGE
Target Name Siglec 15 (ECD)
Species Human
A.A. Sequence Phe20 - Thr263 of Human Siglec 15 (NP_998767.1) with 6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured by its ability to inhibit Anti-CD3-induced proliferation of Jurkat cells. The ED50 for this effect is 4.99-19.98 µg/ml.
Alternate Names HsT1361; CD33 antigen-like 3; SIGLEC-15; CD33L3; Sialic acid-binding Ig-like lectin 15; Siglec-15

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 300 mM NaCl.
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 284266 Human SIGLEC15

Swiss-port # Q6ZMC9 Human Sialic acid-binding Ig-like lectin 15

Gene Symbol SIGLEC15
Gene Full Name sialic acid binding Ig-like lectin 15
Function Binds sialylated glycoproteins. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 36 kDa