ARG70342
Human Siglec 15 recombinant protein (Active) (ECD) (His-tagged, C-ter)
Human Siglec 15 recombinant protein (Active) (ECD) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Active Human Siglec 15 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt, SDS-PAGE |
| Target Name | Siglec 15 (ECD) |
| Species | Human |
| A.A. Sequence | Phe20 - Thr263 of Human Siglec 15 (NP_998767.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Measured by its ability to inhibit Anti-CD3-induced proliferation of Jurkat cells. The ED50 for this effect is 4.99-19.98 µg/ml. |
| Alternate Names | HsT1361; CD33 antigen-like 3; SIGLEC-15; CD33L3; Sialic acid-binding Ig-like lectin 15; Siglec-15 |
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 300 mM NaCl. |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6ZMC9 Human Sialic acid-binding Ig-like lectin 15 |
|---|---|
| Gene Symbol | SIGLEC15 |
| Gene Full Name | sialic acid binding Ig-like lectin 15 |
| Function | Binds sialylated glycoproteins. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 36 kDa |
