ARG83634
Human Siglec 5 + Siglec 14 ELISA kit
Human Siglec 5 + Siglec 14 ELISA kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG83634-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83634-02 | Standard (Lyophilized) | 3 vials | 4°C |
| ARG83634-03 | Standard diluent buffer | 20 ml | 4°C |
| ARG83634-04 | Antibody conjugate concentrate | 1 vials | 4°C |
| ARG83634-05 | Antibody diluent buffer | 16 ml | 4°C |
| ARG83634-06 | HRP-Streptavidin concentrate | 1 vials | 4°C (protect from light) |
| ARG83634-07 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG83634-08 | 20X Wash buffer | 50 ml | 4°C |
| ARG83634-09 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83634-10 | STOP solution | 12 ml | 4°C |
| ARG83634-11 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG83634 Human Siglec 5 + Siglec 14 ELISA kit for the quantification of Human Siglec 5 + Siglec 14 in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Siglec 3/Fc Chimera ; Siglec 7/Fc Chimera ; Siglec 9/Fc Chimera |
| Target Name | Siglec 5 + Siglec 14 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.5 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | OB-BP2 ; SIGLEC-5 ; CD33L2 ; CD170 ; Sialic Acid-Binding Ig-Like Lectin 5 ; Obesity-Binding Protein 2 ; OB-Binding Protein 2 ; CD33 Antigen-Like 2 ; OBBP2 ; Sialic Ac ; d-Binding Immunoglobulin-Like Lectin 5 ; Sialic Acid Binding Ig-Like Lectin 5 ; CD170 Antigen ; Siglec-5 / Sialic Acid-Binding Ig-Like Lectin 14 ; Siglec-14 |
Application Instructions
| Assay Time | ~ 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SIGLEC5 + SIGLEC14 |
|---|---|
| Gene Full Name | Sialic Acid Binding Ig Like Lectin 5 + Sialic Acid Binding Ig Like Lectin 14 |
| Background | Siglec 5 encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012] Siglec 14 predicted to enable sialic acid binding activity. Predicted to be involved in cell adhesion. Predicted to be located in ficolin-1-rich granule membrane and tertiary granule membrane. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Dec 2024] |
| Function | Siglec 5:Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt]] Siglec 14:Putative adhesion molecule. Sialic acid-binding paired receptor which may activate associated receptors. [UniProt] |
| Cellular Localization | Membrane [UniProt] |
| Highlight | Related products: Siglec antibodies; Siglec ELISA Kits; Siglec Duos / Panels New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Disulfide bond, Glycoprotein [UniProt] |
| Title | Download Link |
|---|---|
| ARG83634 Human Siglec 5 + Siglec 14 ELISA kit User's manual |
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