ARG82845
Human Smac / Diablo ELISA Kit
Human Smac / Diablo ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82845-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82845-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82845-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82845-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82845-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82845-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82845-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82845-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82845-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82845-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82845-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82845 Human Smac / Diablo ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Smac / Diablo in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Smac / Diablo |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 7.6% |
| Alternate Names | Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DIABLO |
| Gene Full Name | diablo, IAP-binding mitochondrial protein |
| Background | This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] |
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. [UniProt] |
| Cellular Localization | Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis. [UniProt] |
| PTM | Ubiquitinated by BIRC7/livin. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82845 Human Smac / Diablo ELISA Kit User manual |
 Download
|
