ARG80123

Human TGF beta 1 ELISA Kit

Publication2

Ordering Information

Package

Price (USD)

96 wells

425.00

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Component

Cat No	Component Name	Package	Temp
ARG80123-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80123-002	Standard (Lyophilized)	3 X 1.8 ng/vial	4°C
ARG80123-003	Standard diluent buffer	20 ml	4°C
ARG80123-004	Antibody conjugate concentrate	400 µl	4°C
ARG80123-005	Antibody diluent buffer	16 ml	4°C
ARG80123-006	HRP-Streptavidin concentrate	400 µl	4°C (Protect from light)
ARG80123-007	HRP-Streptavidin diluent buffer	16 ml	4°C
ARG80123-008	20X Wash buffer	50 ml	4°C
ARG80123-009	TMB substrate	12ml	4°C (Protect from light)
ARG80123-010	STOP solution	12ml	4°C
ARG80123-011	1N HCl	5 ml	4°C
ARG80123-012	1N NaOH	5 ml	4°C
ARG80123-013	Plate sealer	4 strips	Room temperature

Overview

Product Description	ARG80123 Human TGF beta 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TGF beta 1 in Serum, Plasma, Cell culture supernatants.
Tested Reactivity	Hu
Tested Application	ELISA
Specificity	No significant cross-reactivity or interference with Human TGF beta 2, TGF beta 3.
Target Name	TGF beta 1
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm
Sensitivity	15 pg/ml
Sample Type	Serum, Plasma, Cell culture supernatants
Standard Range	31.25 - 2000 pg/ml
Sample Volume	100 µl
Precision	CV: <10%
Alternate Names	TGFB; DPD1; TGFbeta; CED; Transforming growth factor beta-1; LAP; TGF-beta-1

Application Instructions

Assay Time	4 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol	TGFB1
Gene Full Name	transforming growth factor, beta 1
Background	This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009]
Function	Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. [UniProt]
Highlight	Related products: TGF beta1 antibodies; TGF beta1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData
Research Area	Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit
PTM	Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

Specific References

Incomplete Recovery from the Radiocontrast-Induced Dysregulated Cell Cycle, Adhesion, and Fibrogenesis in Renal Tubular Cells after Radiocontrast (Iohexol) Removal

ELISA / Human/Cell culture supernatants

Hsing-Yu Chen et al.
Int J Mol Sci., (2023)