ARG81495
Human TGF beta 2 ELISA Kit
Human TGF beta 2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81495-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81495-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81495-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81495-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81495-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81495-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81495-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81495-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81495-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81495-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81495-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81495 Human TGF beta 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TGF beta 2 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Bov, Chk, Dog, Hrs, Mk, Rb |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TGF beta 2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.6% Inter-Assay CV: 7.4% |
| Alternate Names | TGF-beta2; Cetermin; LDS4; Polyergin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Transforming growth factor beta-2; LAP; TGF-beta-2; BSC-1 cell growth inhibitor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TGFB2 |
| Gene Full Name | transforming growth factor, beta 2 |
| Background | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
| Function | TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. [UniProt] |
| Highlight | Related products: TGF beta antibodies; TGF beta ELISA Kits; TGF beta recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81495 Human TGF beta 2 ELISA Kit User manual |
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