ARG81522
Human TGFBI ELISA Kit
Human TGFBI ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81522-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81522-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81522-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81522-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81522-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81522-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81522-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81522-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81522-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81522-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81522-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81522 Human TGFBI ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TGFBI in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TGFBI |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 6.5% |
| Alternate Names | CDGG1; LCD1; RGD-CAP; CSD2; CSD; Beta ig-h3; CSD1; Transforming growth factor-beta-induced protein ig-h3; RGD-containing collagen-associated protein; BIGH3; CDG2; CSD3; Kerato-epithelin; CDB1; EBMD |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15582 Human Transforming growth factor-beta-induced protein ig-h3 |
|---|---|
| Gene Symbol | TGFBI |
| Gene Full Name | transforming growth factor, beta-induced, 68kDa |
| Background | This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008] |
| Function | Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. [UniProt] |
| Highlight | Related products: TGFBI antibodies; TGFBI ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81522 Human TGFBI ELISA Kit User's manual |
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