ARG82596

Human TIE1 ELISA Kit

Human TIE1 ELISA Kit for ELISA and Human

Overview

Product Description ARG82596 Human TIE1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIE1 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This kit could assay both natural and recombinant Human TIE1.

No significant cross-reactivity or interference was observed in the following samples:
Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF.
Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha.
Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha.
Target Name TIE1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.16 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.31 - 20 ng/ml
Sample Volume 20 µl
Precision Intra-Assay CV: 3.6%
Inter-Assay CV: 2.5%
Alternate Names TIE; Tyrosine-protein kinase receptor Tie-1; JTK14; EC 2.7.10.1

Application Instructions

Assay Time ~ 2.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7075 Human TIE1

Swiss-port # P35590 Human Tyrosine-protein kinase receptor Tie-1

Gene Symbol TIE1
Gene Full Name tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Background This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Function Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
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PTM Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. [UniProt]
Title Download Link
ARG82596 Human TIE1 ELISA Kit User manual download icon Download