ARG70241

Human TIGIT recombinant protein (ECD) (His-tagged, C-ter)

Human TIGIT recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Human TIGIT recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name TIGIT (ECD)
Species Human
A.A. Sequence Met22 - Pro141 of Human TIGIT (NP_776160.2) with 6X His tag at the C-terminus.
Expression System HEK293
Alternate Names WUCAM; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; T-cell immunoreceptor with Ig and ITIM domains

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT at 1.5 ug/ml (100 µl/well) can bind Human CD155 with a linear range of 0.15-1.2ug/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 201633 Human TIGIT

Swiss-port # Q495A1 Human T-cell immunoreceptor with Ig and ITIM domains

Gene Symbol TIGIT
Gene Full Name T cell immunoreceptor with Ig and ITIM domains
Background This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [provided by RefSeq, Sep 2009]
Function Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 26 kDa