ARG70241
Human TIGIT recombinant protein (ECD) (His-tagged, C-ter)
Human TIGIT recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human TIGIT recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | TIGIT (ECD) |
| Species | Human |
| A.A. Sequence | Met22 - Pro141 of Human TIGIT (NP_776160.2) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | WUCAM; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; T-cell immunoreceptor with Ig and ITIM domains |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT at 1.5 ug/ml (100 µl/well) can bind Human CD155 with a linear range of 0.15-1.2ug/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q495A1 Human T-cell immunoreceptor with Ig and ITIM domains |
|---|---|
| Gene Symbol | TIGIT |
| Gene Full Name | T cell immunoreceptor with Ig and ITIM domains |
| Background | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [provided by RefSeq, Sep 2009] |
| Function | Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 26 kDa |
