ARG81989

Human TIM3 ELISA Kit

Human TIM3 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81989-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81989-002 Standard 2 X 10 ng/vial 4°C
ARG81989-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81989-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81989-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81989-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81989-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81989-008 25X Wash buffer 20 ml 4°C
ARG81989-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81989-010 STOP solution 10 ml (Ready to use) 4°C
ARG81989-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81989 Human TIM3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIM3 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name TIM3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.1%
Alternate Names KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84868 Human HAVCR2

Swiss-port # Q8TDQ0 Human Hepatitis A virus cellular receptor 2

Gene Symbol HAVCR2
Gene Full Name hepatitis A virus cellular receptor 2
Background The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Function Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt]
Highlight Related products:
TIM3 antibodies; TIM3 ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM O-glycosylated with core 1 or possibly core 8 glycans.

Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9. [UniProt]
Title Download Link
ARG81989 Human TIM3 ELISA Kit User manual download icon Download