ARG80187

Human TIMP1 ELISA Kit

Human TIMP1 ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80187-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80187-002 Standard (Lyophilized) 2 X 1 ng/vial 4°C
ARG80187-003 Standard diluent buffer 20 ml 4°C
ARG80187-004 Antibody conjugate concentrate 400 µl 4°C
ARG80187-005 Antibody diluent buffer 16 ml 4°C
ARG80187-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80187-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80187-008 20X Wash buffer 50 ml 4°C
ARG80187-009 TMB substrate 12ml 4°C (Protect from light)
ARG80187-010 STOP solution 12ml 4°C
ARG80187-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80187 Human TIMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIMP1 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human MMP-1, MMP-3, MMP-2, TIMP-2;mouse TIMP-1
Target Name TIMP1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 16 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Erythroid-potentiating activity; TIMP; Collagenase inhibitor; Fibroblast collagenase inhibitor; TIMP-1; EPO; CLGI; Tissue inhibitor of metalloproteinases 1; HCI; Metalloproteinase inhibitor 1; EPA

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7076 Human TIMP1

Swiss-port # P01033 Human Metalloproteinase inhibitor 1

Gene Symbol TIMP1
Gene Full Name TIMP metallopeptidase inhibitor 1
Background This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq]
Highlight Related products:
TIMP1 antibodies; TIMP1 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
PTM The activity of TIMP1 is dependent on the presence of disulfide bonds.
N-glycosylated.
Title Download Link
ARG80187 Human TIMP1 ELISA Kit User's manual download icon Download