ARG70269

Human TNFRSF11B / OPG recombinant protein (His-tagged, C-ter)

Human TNFRSF11B / OPG recombinant protein (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Human TNFRSF11B / OPG recombinant protein.
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name TNFRSF11B / OPG
Species Human
A.A. Sequence Glu22 - Leu401 of Human TNFRSF11B / OPG (NP_002537.3) with 6X His tag at the C-terminus.
Expression System HEK293
Alternate Names OCIF; Osteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; PDB5; Osteoprotegerin; OPG; TR1

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant human TNFRSF11B at 2 µg/ml (100 µl/well) can bind Recombinant human TNFSF11 with a linear range of 2-8 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4982 Human TNFRSF11B

Swiss-port # O00300 Human Tumor necrosis factor receptor superfamily member 11B

Gene Symbol TNFRSF11B
Gene Full Name tumor necrosis factor receptor superfamily, member 11b
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Function Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 46 kDa
PTM N-glycosylated. Contains sialic acid residues.

The N-terminus is blocked. [UniProt]