ARG82333
Human TNFSF13 / APRIL ELISA Kit
Human TNFSF13 / APRIL ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82333-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82333-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82333-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82333-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82333-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82333-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82333-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82333-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82333-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82333-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82333-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82333 Human TNFSF13 / APRIL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TNFSF13 / APRIL in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | TNFSF13 / APRIL |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 7.2% |
| Alternate Names | TALL2; ZTNF2; TRDL-1; TNF- and APOL-related leukocyte expressed ligand 2; CD256; APRIL; A proliferation-inducing ligand; TNF-related death ligand 1; UNQ383/PRO715; CD antigen CD256; Tumor necrosis factor ligand superfamily member 13; TALL-2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75888 Human Tumor necrosis factor ligand superfamily member 13 |
|---|---|
| Gene Symbol | TNFSF13 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 13 |
| Background | The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010] |
| Function | Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: TNFSF antibodies; TNFSF ELISA Kits; TNFSF Duos / Panels; TNFSF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The precursor is cleaved by furin. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82333 Human TNFSF13 / APRIL ELISA kit User manual |
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