ARG70085
Human TNFSF18 / GITRL recombinant protein (Active) (His-tagged, C-ter)
Human TNFSF18 / GITRL recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human TNFSF18 / GITRL recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | TNFSF18 / GITRL |
| Species | Human |
| A.A. Sequence | MQLET AKEPC MAKFG PLPSK WQMAS SEPPC VNKVS DWKLE ILQNG LYLIY GQVAP NANYN DVAPF EVRLY KNKDM IQTLT NKSKI QNVGG TYELH VGDTI DLIFN SEHQV LKNNT YWGII LIAN PQEI with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 2.5 ng/ml. |
| Alternate Names | TL6; AITRL; Glucocorticoid-induced TNF-related ligand; Tumor necrosis factor ligand superfamily member 18; hGITRL; Activation-inducible TNF-related ligand; GITRL |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNFSF18 |
|---|---|
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 18 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1. Promotes leukocyte adhesion to endothelial cells. Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. [UniProt] |
