ARG80182

Human Thrombopoietin (Tpo) ELISA Kit

Human Thrombopoietin (Tpo) ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit

Component

Cat No Component Name Package Temp
ARG80182-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80182-002 Standard (Lyophilized) 3 X 4 ng/vial 4°C
ARG80182-003 Standard diluent buffer 20 ml 4°C
ARG80182-004 Antibody conjugate concentrate 400 µl 4°C
ARG80182-005 Antibody diluent buffer 16 ml 4°C
ARG80182-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80182-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80182-008 20X Wash buffer 50 ml 4°C
ARG80182-009 TMB substrate 12ml 4°C (Protect from light)
ARG80182-010 STOP solution 12ml 4°C
ARG80182-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80182 Human Thrombopoietin (Tpo) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Thrombopoietin (Tpo) in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human Epo, Epo R/Fc Chimera, Tpo R/Fc Chimera;mouse Epo, Tpo
Target Name Tpo / Thrombopoietin
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 62 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names C-mpl ligand; Thrombopoietin; MPLLG; TPO; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; MKCSF; THCYT1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7066 Human THPO

Swiss-port # P40225 Human Thrombopoietin

Gene Symbol THPO
Gene Full Name thrombopoietin
Background TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant murine TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Highlight Related products:
Thrombopoietin antibodies; Thrombopoietin ELISA Kits; Thrombopoietin recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit
Title Download Link
ARG80182 Human Thrombopoietin (Tpo) ELISA Kit User's manual download icon Download