ARG81484

Human Tissue Factor ELISA Kit

Human Tissue Factor ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81484-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81484-002 Standard 2 X 10 ng/vial 4°C
ARG81484-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81484-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81484-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81484-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81484-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81484-008 25X Wash buffer 20 ml 4°C
ARG81484-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81484-010 STOP solution 10 ml (Ready to use) 4°C
ARG81484-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81484 Human Tissue Factor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Tissue Factor in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Tissue Factor
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%
Inter-Assay CV: 6.9%
Alternate Names Thromboplastin; Tissue factor; TFA; CD142; TF; Coagulation factor III; CD antigen CD142

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2152 Human F3

Swiss-port # P13726 Human Tissue factor

Gene Symbol F3
Gene Full Name coagulation factor III (thromboplastin, tissue factor)
Background This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Function Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. [UniProt]
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Tissue Factor antibodies; Tissue Factor ELISA Kits;
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Title Download Link
ARG81484 Human Tissue Factor ELISA Kit User's manual download icon Download