ARG70139
Human Tpo / Thrombopoietin recombinant protein (Active) (His-tagged, N-ter)
Human Tpo / Thrombopoietin recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human Tpo / Thrombopoietin recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Tpo / Thrombopoietin |
| Species | Human |
| A.A. Sequence | SPAPP ACDLR VLSKL LRDSH VLHSR LSQCP EVHPL PTPVL LPAVD FSLGE WKTQM EETKA QDILG AVTLL LEGVM AARGQ LGPTC LSSLL GQLSG QVRLL LGALQ SLLGT QLPPQ GRTTA HKDPN AIFLS FQHLL RGKVR FLMLV GGSTL CVRRA PPTTA VPSRT SLVLT LNEL with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in MO7e cells. The ED50 for this effect is < 2 ng/ml. The specific activity of recombinant human TPO is > 5 x 10^5 IU/mg. |
| Alternate Names | C-mpl ligand; Thrombopoietin; MPLLG; TPO; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; MKCSF; THCYT1 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | THPO |
|---|---|
| Gene Full Name | thrombopoietin |
| Background | Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014] |
| Function | Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
