ARG82368

Human ULBP1 ELISA Kit

Human ULBP1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82368-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82368-002 Standard 2 X 10 ng/vial 4°C
ARG82368-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82368-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82368-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82368-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82368-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82368-008 25X Wash buffer 20 ml 4°C
ARG82368-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82368-010 STOP solution 10 ml (Ready to use) 4°C
ARG82368-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82368 Human ULBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ULBP1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name ULBP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.85 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 93.7 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%
Inter-Assay CV: 7.9%
Alternate Names UL16-binding protein 1; Retinoic acid early transcript 1I; RAET1I; N2DL-1; NKG2DL1; NKG2D ligand 1; ALCAN-beta

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80329 Human ULBP1

Swiss-port # Q9BZM6 Human NKG2D ligand 1

Gene Symbol ULBP1
Gene Full Name UL16 binding protein 1
Function Ligand for the KLRK1/NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the KLRK1/NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. Endoplasmic reticulum. Note=In CMV-infected fibroblasts, detected in the endoplasmic reticulum/cis-Golgi. [UniProt]
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Title Download Link
ARG82368 Human ULBP1 ELISA Kit User manual download icon Download