ARG81611
Human ULBP2 ELISA Kit
Human ULBP2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81611-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81611-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81611-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81611-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81611-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81611-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81611-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81611-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81611-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81611-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81611-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81611 Human ULBP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ULBP2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ULBP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 7.1% |
| Alternate Names | N2DL-2; Retinoic acid early transcript 1H; RAET1H; NKG2DL2; UL16-binding protein 2; N2DL2; ALCAN-alpha; NKG2D ligand 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ULBP2 |
| Gene Full Name | UL16 binding protein 2 |
| Background | This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015] |
| Function | Ligand for the KLRK1/NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the KLRK1/NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. [UniProt] |
| Highlight | Related products: ULBP antibodies; ULBP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG81611 Human ULBP2 ELISA Kit User's manual |
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