ARG81611

Human ULBP2 ELISA Kit

Human ULBP2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81611-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81611-002 Standard 2 X 10 ng/vial 4°C
ARG81611-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81611-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81611-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81611-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81611-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81611-008 25X Wash buffer 20 ml 4°C
ARG81611-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81611-010 STOP solution 10 ml (Ready to use) 4°C
ARG81611-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81611 Human ULBP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ULBP2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ULBP2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.1%
Alternate Names N2DL-2; Retinoic acid early transcript 1H; RAET1H; NKG2DL2; UL16-binding protein 2; N2DL2; ALCAN-alpha; NKG2D ligand 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80328 Human ULBP2

Swiss-port # Q9BZM5 Human NKG2D ligand 2

Gene Symbol ULBP2
Gene Full Name UL16 binding protein 2
Background This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]
Function Ligand for the KLRK1/NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the KLRK1/NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. [UniProt]
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Title Download Link
ARG81611 Human ULBP2 ELISA Kit User's manual download icon Download