ARG80134

Human VCAM1 ELISA Kit

Human VCAM1 ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
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Component

Cat No Component Name Package Temp
ARG80134-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80134-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80134-003 Standard diluent buffer 20 ml 4°C
ARG80134-004 Antibody conjugate concentrate 400 µl 4°C
ARG80134-005 Antibody diluent buffer 16 ml 4°C
ARG80134-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80134-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80134-008 20X Wash buffer 50 ml 4°C
ARG80134-009 TMB substrate 12ml 4°C (Protect from light)
ARG80134-010 STOP solution 12ml 4°C
ARG80134-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80134 Human VCAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VCAM1 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human E-Selectin/Fc Chimera , ICAM-1, ICAM-2/Fc Chimera, ICAM-3/Fc Chimera, MCAM and NCAM-L1/Fc Chimera; Mouse DCC/Fc Chimera and VCAM-1/Fc Chimera.
Target Name CD106 / VCAM1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15.6 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision CV: < 10%
Alternate Names CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol VCAM1
Gene Full Name vascular cell adhesion molecule 1
Background This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]
Highlight Related products:
CD106 antibodies; CD106 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
PTM Sialoglycoprotein.

Specific References

Prognostic value of serum levels of multiple adhesion factors in patients with sepsis-induced acute kidney injury

ELISA / Human / Serum

Yan Li et al.
Int Urol Nephrol,  (2022)

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Title Download Link
ARG80134 Human VCAM1 ELISA Kit User's manual download icon Download