ARG80134
Human VCAM1 ELISA Kit
Human VCAM1 ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80134-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80134-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80134-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80134-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80134-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80134-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80134-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80134-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80134-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80134-010 | STOP solution | 12ml | 4°C |
| ARG80134-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80134 Human VCAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VCAM1 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human E-Selectin/Fc Chimera , ICAM-1, ICAM-2/Fc Chimera, ICAM-3/Fc Chimera, MCAM and NCAM-L1/Fc Chimera; Mouse DCC/Fc Chimera and VCAM-1/Fc Chimera. |
| Target Name | CD106 / VCAM1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: < 10% |
| Alternate Names | CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | VCAM1 |
|---|---|
| Gene Full Name | vascular cell adhesion molecule 1 |
| Background | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010] |
| Function | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt] |
| Highlight | Related products: CD106 antibodies; CD106 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit |
| PTM | Sialoglycoprotein. |
Specific References
Prognostic value of serum levels of multiple adhesion factors in patients with sepsis-induced acute kidney injury
ELISA / Human / Serum
| Title | Download Link |
|---|---|
| ARG80134 Human VCAM1 ELISA Kit User's manual |
 Download
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