ARG81885

Human VE Cadherin ELISA Kit

Human VE Cadherin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81885-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81885-002 Standard 2 X 20 ng/vial 4°C
ARG81885-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81885-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81885-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81885-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81885-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81885-008 25X Wash buffer 20 ml 4°C
ARG81885-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81885-010 STOP solution 10 ml (Ready to use) 4°C
ARG81885-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81885 Human VE Cadherin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VE Cadherin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name VE Cadherin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.2%; Inter-Assay CV: 8.6%
Alternate Names 7B4 antigen; 7B4; Cadherin-5; VE-cadherin; CD144; CD antigen CD144; Vascular endothelial cadherin

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1003 Human CDH5

Swiss-port # P33151 Human Cadherin-5

Gene Symbol CDH5
Gene Full Name cadherin 5, type 2 (vascular endothelium)
Background This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. [UniProt]
Highlight Related products:
Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity).

O-glycosylated. [UniProt]
Title Download Link
ARG81885 Human VE Cadherin ELISA Kit User's manual download icon Download