ARG81305

Human VEGF165 ELISA Kit

Human VEGF165 ELISA Kit for ELISA and Human

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Overview

Product Description

ARG81305 Human VEGF165 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human VEGF165 in serum, plasma and cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No cross reaction with VEGF-121, VEGF-145, VEGF-B, VEGF-C, VEGF-D, VEGF-R1, Ang-2, GM-CSF, IFN gamma, IL-29. Shows cross reactivity with VEGF-189 isoform.
Target Name VEGF165
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 15.9 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 1000 pg/ml
Sample Volume 50 µl
Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7422 Human VEGFA

Swiss-port # P15692 Human Vascular endothelial growth factor A

Gene Symbol VEGFA
Gene Full Name vascular endothelial growth factor A
Background This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [UniProt]
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Specific References

FOXM1D potentiates PKM2-mediated tumor glycolysis and angiogenesis

ELISA / Human

Wei Zhang et al.
Mol Oncol.,  (2021)

publication_link

 

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