ARG82830
Human alpha 1 Antitrypsin ELISA Kit
Human alpha 1 Antitrypsin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82830-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82830-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG82830-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82830-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82830-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82830-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82830-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82830-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82830-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82830-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82830-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82830 Human alpha 1 Antitrypsin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha 1 Antitrypsin in serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | alpha 1 Antitrypsin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.78 ng/ml |
| Sample Type | Serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.0% Inter-Assay CV: 7.9% |
| Alternate Names | Serpin A1; alpha1AT; Alpha-1-antiproteinase; Alpha-1 protease inhibitor; AAT; A1A; A1AT; PRO2275; Alpha-1-antitrypsin; PI1; PI; SPAAT |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPINA1 |
| Gene Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
| Background | The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. [Short peptide from AAT]: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). [UniProt] |
| Cellular Localization | Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum. Short peptide from AAT: Secreted, extracellular space, extracellular matrix. [UniProt] |
| PTM | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82830 Human alpha 1 Antitrypsin ELISA Kit User manual |
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