ARG81612

Human alpha 1 microglobulin ELISA Kit

Human alpha 1 microglobulin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81612-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81612-002 Standard 2 X 10 ng/vial 4°C
ARG81612-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81612-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81612-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81612-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81612-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81612-008 25X Wash buffer 20 ml 4°C
ARG81612-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81612-010 STOP solution 10 ml (Ready to use) 4°C
ARG81612-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81612 Human alpha 1 microglobulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha 1 microglobulin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name alpha 1 microglobulin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 6.7%
Alternate Names Bikunin; HCP; ITIL; ITILC; ITI; ITI-LC; UTI; Protein HC; Complex-forming glycoprotein heterogeneous in charge; EDC1; HI30; HI-30; Alpha-1 microglycoprotein; A1M; IATIL; Uronic-acid-rich protein; Protein AMBP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 259 Human AMBP

Swiss-port # P02760 Human Protein AMBP

Gene Symbol AMBP
Gene Full Name alpha-1-microglobulin/bikunin precursor
Background This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]
Function Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Trypstatin is a trypsin inhibitor. [UniProt]
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PTM The precursor is proteolytically processed into separately functioning proteins.

3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.

Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.

N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains. [UniProt]
Title Download Link
ARG81612 Human alpha 1 microglobulin ELISA Kit User's manual download icon Download