ARG83218
Human alpha 2 Macroglobulin ELISA Kit (Rapid One-Step)
Human alpha 2 Macroglobulin ELISA Kit (Rapid One-Step) for ELISA and Human
Overview
| Product Description | ARG83218 Human alpha 2 Macroglobulin ELISA Kit (Rapid One-Step) is an Enzyme Immunoassay kit for the quantification of Human alpha 2 Macroglobulin in Serum, Plasma, Cell lysates and Cell culture supernatants. It is a rapid One-step 90 minutes protocol. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | alpha 2 Macroglobulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 300 pg/ml |
| Detection Range | 625 pg/ml - 40,000 pg/ml |
| Sample Type | Serum, Plasma, Cell lysates and Cell culture supernatants |
| Precision | Intra-Assay CV: 6.8% Inter-Assay CV: 7.3% |
| Alternate Names | CPAMD5; Alpha-2-macroglobulin; S863-7; FWP007; Alpha-2-M; A2MD; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 |
Application Instructions
| Assay Time | ~ 1.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | A2M |
| Gene Full Name | alpha-2-macroglobulin |
| Background | Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008] |
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Title | Download Link |
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| ARG83218 Human alpha 2 Macroglobulin ELISA Kit (Rapid One-Step) User manual |
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