ARG82004

Human alpha Defensin 1 ELISA Kit

Human alpha Defensin 1 ELISA Kit for ELISA and Human

publication_link Publication1

Component

Cat No Component Name Package Temp
ARG82004-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82004-002 Standard 2 X 10 ng/vial 4°C
ARG82004-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82004-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82004-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82004-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82004-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82004-008 25X Wash buffer 20 ml 4°C
ARG82004-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82004-010 STOP solution 10 ml (Ready to use) 4°C
ARG82004-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82004 Human alpha Defensin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Defensin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross Reactivity: There is no detectable cross-reactivity with recombinant human α-Defensin 5, β-Defensin 3, C1qB, MCP-2 and Serpin F2 and the kit might also react to HNP2 and HNP3.
Target Name alpha Defensin 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 5.5%
Alternate Names DEF1; Neutrophil defensin 1; HNP-1; HNP-2; HP-2; HP1; Defensin, alpha 1; MRS; DEFA2; HP2; HP-1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1667 Human DEFA1

Swiss-port # P59665 Human Neutrophil defensin 1

Gene Symbol DEFA1
Gene Full Name defensin, alpha 1
Background Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014]
Function Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. [UniProt]
Cellular Localization Secreted. [UniProt]
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Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation. [UniProt]

Specific References

Serum proteins may facilitate the identification of Kawasaki disease and promote in vitro neutrophil infiltration.

ELISA / Human / Serum

Sung-Chou Li et al.
Sci Rep.,  (2020)

publication_link

 

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ARG82004 Human alpha Defensin 1 ELISA Kit User's manual download icon Download