ARG82004
Human alpha Defensin 1 ELISA Kit
Human alpha Defensin 1 ELISA Kit for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82004-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82004-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82004-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82004-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82004-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82004-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82004-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82004-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82004-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82004-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82004-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82004 Human alpha Defensin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Defensin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross Reactivity: There is no detectable cross-reactivity with recombinant human α-Defensin 5, β-Defensin 3, C1qB, MCP-2 and Serpin F2 and the kit might also react to HNP2 and HNP3. |
| Target Name | alpha Defensin 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2%; Inter-Assay CV: 5.5% |
| Alternate Names | DEF1; Neutrophil defensin 1; HNP-1; HNP-2; HP-2; HP1; Defensin, alpha 1; MRS; DEFA2; HP2; HP-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DEFA1 |
| Gene Full Name | defensin, alpha 1 |
| Background | Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014] |
| Function | Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: alpha Defensin 1 antibodies; alpha Defensin 1 ELISA Kits; Related news: New MMP7 (total or active) ELISA kits are released New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production. Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation. [UniProt] |
Specific References
Serum proteins may facilitate the identification of Kawasaki disease and promote in vitro neutrophil infiltration.
ELISA / Human / Serum
| Title | Download Link |
|---|---|
| ARG82004 Human alpha Defensin 1 ELISA Kit User's manual |
 Download
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