ARG70247

Human beta 2 Microglobulin recombinant protein (His-tagged, C-ter)

Human beta 2 Microglobulin recombinant protein (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Human beta 2 Microglobulin recombinant protein.
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name beta 2 Microglobulin
Species Human
A.A. Sequence Ile21 - Met119 of Human beta 2 Microglobulin (NP_004039.1) with 6X His tag at the C-terminus.
Expression System HEK293
Alternate Names Beta-2-microglobulin

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B2M at 2µg/ml (100µl/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 567 Human B2M

Swiss-port # P61769 Human Beta-2-microglobulin

Gene Symbol B2M
Gene Full Name beta-2-microglobulin
Background This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. [provided by RefSeq, Aug 2014]
Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553). [UniProt]
Cellular Localization Secreted. Cell surface. Note=Detected in serum and urine (PubMed:1336137, PubMed:7554280). Note=(Microbial infection) In the presence of M.tuberculosis EsxA-EsxB complex decreased amounts of B2M are found on the cell surface (PubMed:25356553). [UniProt]
Calculated MW 14 kDa
PTM Glycation of Ile-21 is observed in long-term hemodialysis patients. [UniProt]