ARG70247
Human beta 2 Microglobulin recombinant protein (His-tagged, C-ter)
Human beta 2 Microglobulin recombinant protein (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human beta 2 Microglobulin recombinant protein. |
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| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | beta 2 Microglobulin |
| Species | Human |
| A.A. Sequence | Ile21 - Met119 of Human beta 2 Microglobulin (NP_004039.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | Beta-2-microglobulin |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B2M at 2µg/ml (100µl/well) can bind Recombinant Human CD8 alpha with a linear range of 31.25-341 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | B2M |
| Gene Full Name | beta-2-microglobulin |
| Background | This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. [provided by RefSeq, Aug 2014] |
| Function | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553). [UniProt] |
| Cellular Localization | Secreted. Cell surface. Note=Detected in serum and urine (PubMed:1336137, PubMed:7554280). Note=(Microbial infection) In the presence of M.tuberculosis EsxA-EsxB complex decreased amounts of B2M are found on the cell surface (PubMed:25356553). [UniProt] |
| Calculated MW | 14 kDa |
| PTM | Glycation of Ile-21 is observed in long-term hemodialysis patients. [UniProt] |
