ARG82268

Human beta Endorphin ELISA Kit

Human beta Endorphin ELISA Kit for ELISA and Human

Overview

Product Description ARG82268 Human beta Endorphin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human beta Endorphin in plasma.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross-Reactivity:
beta Endorphin (Human) and Ac-beta Endorphin (Human): 100%
beta Endorphin (Rat), Met-Enkephalin, alpha MSH and ACTH (Human): 0%
Target Name beta Endorphin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.125 ng/ml
Sample Type Plasma
Standard Range 0.01 - 100 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 15%
Alternate Names Alpha-MSH; Beta-MSH; CLIP; Gamma-MSH; LPH; Corticotropin-lipotropin; NPP; ACTH; POMC; Gamma-LPH; Adrenocorticotropic hormone; MSH; Beta-LPH; Pro-opiomelanocortin; POC

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5443 Human POMC

Swiss-port # P01189 Human Pro-opiomelanocortin

Gene Symbol POMC
Gene Full Name proopiomelanocortin
Background This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Nov 2014]
Function ACTH stimulates the adrenal glands to release cortisol.

MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.

Beta-endorphin and Met-enkephalin are endogenous opiates. [UniProt]
Cellular Localization Secreted. Note=Melanocyte-stimulating hormone alpha and beta-endorphin are stored in separate granules in hypothalamic POMC neurons, suggesting that secretion may be under the control of different regulatory mechanisms. [UniProt]
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PTM Specific enzymatic cleavages at paired basic residues yield the different active peptides.

O-glycosylated; reducing sugar is probably N-acetylgalactosamine. [UniProt]
Title Download Link
ARG82268 Human beta Endorphin ELISA Kit User manual download icon Download