ARG80133

Human beta NGF ELISA Kit

Human beta NGF ELISA Kit for ELISA and Human

Neuroscience kit

Component

Cat No Component Name Package Temp
ARG80133-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80133-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80133-003 Standard diluent buffer 20 ml 4°C
ARG80133-004 Antibody conjugate concentrate 400 µl 4°C
ARG80133-005 Antibody diluent buffer 16 ml 4°C
ARG80133-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80133-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80133-008 20X Wash buffer 50 ml 4°C
ARG80133-009 TMB substrate 12ml 4°C (Protect from light)
ARG80133-010 STOP solution 12ml 4°C
ARG80133-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80133 Human beta NGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human beta NGF in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human Artemin, BDNF, CNTF, EG-VEGF, EGF, NT-3, NT-4, OPG, FGF-9, Galectin-1, GDNF, Midkine, Neurturin, Persephin, Pleiotrophin, RELM- beta, Resistin, VEGF;rat CNTF
Target Name beta NGF
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 4 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names HSAN5; Beta-nerve growth factor; Beta-NGF; NGFB

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol NGF
Gene Full Name nerve growth factor (beta polypeptide)
Background This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
Function Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). [UniProt]
Highlight Related products:
beta NGF antibodies; beta NGF ELISA Kits; beta NGF recombinant proteins;
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Simplify the ELISA analysis by GainData
Research Area Neuroscience kit
Title Download Link
ARG80133 Human beta NGF ELISA Kit User's manual download icon Download