ARG70145
Human beta NGF recombinant protein (Active) (His-tagged, C-ter)
Human beta NGF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human beta NGF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | beta NGF |
| Species | Human |
| A.A. Sequence | MSSSH PIFHR GEFSV CDSVS VWVGD KTTAT DIKGK EVMVL GEVNI NNSVF KQYFF ETKCR DPNPV DSGCR GIDSK HWNSY CTTTH TFVKA LTMDG KQAAW RFIRI DTACV CVLSR KAVRR A with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.7 ng/ml. The specific activity of recombinant human beta-NGF is > 1 x 10^6 IU/mg. |
| Alternate Names | HSAN5; Beta-nerve growth factor; Beta-NGF; NGFB |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 3.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | NGF |
|---|---|
| Gene Full Name | nerve growth factor (beta polypeptide) |
| Background | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
