ARG80130

Human sCD40L ELISA Kit

Human sCD40L ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit

Component

Cat No Component Name Package Temp
ARG80130-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80130-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80130-003 Standard diluent buffer 20 ml 4°C
ARG80130-004 Antibody conjugate concentrate 400 µl 4°C
ARG80130-005 Antibody diluent buffer 16 ml 4°C
ARG80130-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80130-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80130-008 20X Wash buffer 50 ml 4°C
ARG80130-009 TMB substrate 12ml 4°C (Protect from light)
ARG80130-010 STOP solution 12ml 4°C
ARG80130-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80130 Human sCD40L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD40L in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human ANG, AR, BDNF, CD4, CD40, CNTF, CT-1, CTLA-4, Epo, Fas, CDNF, GITR, OPG, OSM
Target Name sCD154 / sCD40L
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 30 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD40LG
Gene Full Name CD40 ligand
Background The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Function Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
Highlight Related products:
CD154 antibodies; CD154 ELISA Kits;
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Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
PTM The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.
Title Download Link
ARG80130 Human sCD40L ELISA Kit User's manual download icon Download