ARG80125

Human sCD54 / sICAM1 ELISA Kit

Human sCD54 / sICAM1 ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
publication_link Publication3

Component

Cat No Component Name Package Temp
ARG80125-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80125-002 Standard (Lyophilized) 3 X 30 ng/vial 4°C
ARG80125-003 Standard diluent buffer 20 ml 4°C
ARG80125-004 Antibody conjugate concentrate 400 µl 4°C
ARG80125-005 Antibody diluent buffer 16 ml 4°C
ARG80125-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80125-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80125-008 20X Wash buffer 50 ml 4°C
ARG80125-009 TMB substrate 12ml 4°C (Protect from light)
ARG80125-010 STOP solution 12ml 4°C
ARG80125-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80125 Human sCD54 / sICAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human sCD54 / sICAM1 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human sCD31, ICAM-2, sE-Selectin, sL-Selectin, sVCAM-1;mouse VCAM-1
Target Name sCD54 / sICAM1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.24 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.47 - 30 ng/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol ICAM1
Gene Full Name intercellular adhesion molecule 1
Background This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt]
Highlight Related products:
CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit; Developmental Biology kit; Immune System kit
PTM Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Specific References

Prognostic value of serum levels of multiple adhesion factors in patients with sepsis-induced acute kidney injury

ELISA / Human / Serum

Yan Li et al.
Int Urol Nephrol,  (2022)

publication_link

 

hr_line

Mechanism of Huashi Xingyu Qingre recipe in treating oral lichen planus based on network pharmacology and clinical trial verification

ELISA / Human / Serum

Deng Qianlan et al.
J Tradit Chin Med.,  (2022)

publication_link

 

hr_line

Testing feasibility of traditional Malay massage compared to relaxation for patients with non-specific low back pain: A pilot randomized-controlled trial.

ELISA / Human / Serum

Nurhanisah Sejari et al.
European Journal of Integrative Medicine.,  (2020)

publication_link

 

hr_line
Title Download Link
ARG80125 Human sICAM1 ELISA Kit User's manual download icon Download