Component

Overview

Product Description	ARG82320 Hydroxyproline (Total Collagen) Assay Kit is a detection kit for the quantification of Hydroxyproline in tissue and cell extracts which can be used as a direct measure of the amount of collagen present.
Tested Reactivity	Hu, Ms, Rat, Mamm
Tested Application	FuncSt
Specificity	The kit is based on the detection of hydroxyproline. Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where its presence increases the triple helix stability. Hydroxyproline is formed posttranslationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.
Target Name	Hydroxyproline (Total Collagen)
Conjugation Note	Read at 570 nm.
Sensitivity	2.5 µg/ml
Sample Type	Tissue and cell extracts.
Standard Range	6.25 - 300 µg/ml
Sample Volume	Tissue: 100 µl Cell extracts: 50 µl
Alternate Names	OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen

Application Instructions

Assay Time	~ 1.5 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1277 Human COL1A1 GeneID: 12842 Mouse COL1A1 GeneID: 29393 Rat COL1A1 Swiss-port # P02452 Human Collagen alpha-1(I) chain Swiss-port # P02454 Rat Collagen alpha-1(I) chain Swiss-port # P11087 Mouse Collagen alpha-1(I) chain More
Gene Symbol	COL1A1
Gene Full Name	collagen, type I, alpha 1
Background	This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Function	Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt]
Cellular Localization	Secreted, extracellular space, extracellular matrix. [UniProt]
PTM	Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt]