ARG81195

Irisin ELISA Kit

Irisin ELISA Kit for ELISA and Dog,Human,Mouse,Rat

Overview

Product Description

ARG81195 Irisin ELISA Kit is a Competitive Enzyme Immunoassay kit for the quantification of Irisin in plasma (EDTA) and serum samples.

Tested Reactivity Hu, Ms, Rat, Dog
Tested Application ELISA
Specificity Irisin, Recombinant (Human, Rat, Mouse, Dog): 100%
Irisin (aa. 1-112) (Human, Mouse, Rat, Dog): 100%
FNDC5, isoform 4 recombinant (Human, Mouse, Rat, Dog): 9%
Irisin precursor, C-terminal 48mer: Human FNDC5 (aa. 165-212); Mouse, Rat FNDC5 (aa. 162-209): 0%
Irisin (aa. 42-95) (Human, Mouse, Rat, Dog): 0%
Target Name Irisin
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 1.91 ng/ml
Detection Range 1.91 - 18.0 ng/ml
Sample Type plasma (EDTA) and serum
Standard Range 0.1 - 1000 ng/ml
Sample Volume 50 µl
Alternate Names irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 252995 Human FNDC5

GeneID: 260327 Rat FNDC5

GeneID: 384061 Mouse FNDC5

Gene Symbol FNDC5
Gene Full Name fibronectin type III domain containing 5
Background This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Function Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt]
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Irisin antibodies; Irisin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.
Title Download Link
ARG81195 Irisin ELISA Kit User's manual download icon Download