ARG81196

Irisin ELISA Kit

Irisin ELISA Kit for ELISA and Dog,Human,Mouse,Rat

publication_link Publication2

Overview

Product Description

ARG81196 Irisin ELISA Kit is a Competitive Enzyme Immunoassay kit for the quantification of Irisin in serum and plasma.

Tested Reactivity Hu, Ms, Rat, Dog
Tested Application ELISA
Specificity Irisin (Human, Mouse, Rat, Canine): 100%
Irisin (aa. 42-112) (Human, Mouse, Rat, Canine): 100%
Irisin, Recombinant (Human, Mouse, Rat, Canine): 100%
FNDC5 (Isoform 4) (Human, Mouse, Rat): 9%
Irisin precursor, C-terminal / FNDC5 (aa. 165 - 212) (Human) / FNDC5 (aa. 162-209) (Mouse, Rat): 0%
Irisin (aa. 42-95) (Human, Mouse, Rat, Canine): 0%
Target Name Irisin
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 6.2 ng/ml
Detection Range Linear Range: 6.2 - 205 ng/ml
Sample Type Serum and plasma.
Standard Range 0.1 - 1000 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 15%
Alternate Names irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 252995 Human FNDC5

GeneID: 260327 Rat FNDC5

GeneID: 384061 Mouse FNDC5

Gene Symbol FNDC5
Gene Full Name fibronectin type III domain containing 5
Background This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Function Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt]
Highlight Related products:
Irisin antibodies; Irisin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.

Specific References

Polysaccharides from Enteromorpha prolifera improves insulin sensitivity and promotes adipose thermogenesis in diet-induced obese mice associated with activation of PGC-1α-FNDC5/irisin pathway

ELISA / Mouse / Serum

FeiXie et al.
Journal of Functional Foods,  (2022)

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Curcumin improves insulin sensitivity and increases energy expenditure in high-fat-diet-induced obese mice associated with activation of FNDC5/irisin

ELISA / Mouse

Tiande Zou et al.
Nutrition .,  (2021)

publication_link

 

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Title Download Link
ARG81196 Irisin ELISA Kit User's manual download icon Download