ARG70214
MERS-CoV nucleocapsid recombinant protein (His-SUMO tagged, N-ter)
MERS-CoV nucleocapsid recombinant protein (His-SUMO tagged, N-ter) for SDS-PAGE and Virus
Publication1
Overview
| Product Description | HEK293 expressed, His-SUMO tagged (N-ter) MERS-CoV nucleocapsid recombinant protein. |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | SDS-PAGE |
| Target Name | MERS-CoV nucleocapsid protein |
| Species | Virus |
| A.A. Sequence | MASPA APRAV SFADN NDITN TNLSR GRGRN PKPRA APNNT VSWYT GLTQH GKVPL TFPPG QGVPL NANST PAQNA GYWRR QDRKI NTGNG IKQLA PRWYF YYTGT GPEAA LPFRA VKDGI VWVHE DGATD APSTF GTRNP NNDSA IVTQF APGTK LPKNF HIEGT GGNSQ SSSRA SSVSR NSSRS SSQGS RSGNS TRGTS PGPSG IGAVG GDLLY LDLLN RLQAL ESGKV KQSQP KVITK KDAAA AKNKM RHKRT STKSF NMVQA FGLRG PGDLQ GNFGD LQLNK LGTED PRWPQ IAELA PTASA FMGMS QFKLT HQNND DHGNP VYFLR YSGAI KLDPK NPNYN KWLEL LEQNI XAYKT FPKKE KKQKA PKEES TDQMS EPPKE QRVQG SITQR TRTRP SVQPG PMIDV NTD with polyhistidine-SUMO tag at the N-terminus. |
| Expression System | HEK293 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Specific References
Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.
Others / Virus
