ARG82770

MPO / Myeloperoxidase Activity Assay Kit (Colorimetric)

MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) for Functional study and All species,Human,Mouse,Rat

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Overview

Product Description ARG82770 MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) can be used to measure Myeloperoxidase activity in whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA).
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name MPO / Myeloperoxidase
Conjugation Note Read at 405 nm (colorimetric)
Sensitivity 4.2 µM (0.63 nmol/well, based on 150 µl volume/well)
Detection Range 4.2 - 333 µM (0.63 - 50 nmol/well, based on 150 µl volume/well)
Sample Type Whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA).
Sample Volume 25 µl
Alternate Names MPO; Myeloperoxidase; EC 1.11.2.2

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time ~ 2.5 hours

Properties

Form Liquid
Storage Instruction Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17523 Mouse MPO

GeneID: 4353 Human MPO

Swiss-port # P05164 Human Myeloperoxidase

Swiss-port # P11247 Mouse Myeloperoxidase

Gene Symbol MPO
Gene Full Name myeloperoxidase
Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Function Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt]
Cellular Localization Lysosome. [UniProt]

Specific References

Hypochlorous Acid Chemistry in Mammalian Cells-Influence on Infection and Role in Various Pathologies

Celia María Curieses Andrés et al.
Int J Mol Sci.,  (2022)

publication_link

 

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