ARG82483

Mouse Apolipoprotein H ELISA Kit

Mouse Apolipoprotein H ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82483-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82483-002 Standard 2 X 10 ng/vial 4°C
ARG82483-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82483-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82483-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82483-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82483-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82483-008 25X Wash buffer 20 ml 4°C
ARG82483-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82483-010 STOP solution 10 ml (Ready to use) 4°C
ARG82483-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82483 Mouse Apolipoprotein H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Apolipoprotein H in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name Apolipoprotein H
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.3%
Alternate Names Apo-H; Beta-2-glycoprotein 1; BG; B2G1; APC inhibitor; Apolipoprotein H; Anticardiolipin cofactor; B2GPI; Beta; 2; Activated protein C-binding protein; B2GP1; Beta-2-glycoprotein I

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11818 Mouse APOH

Swiss-port # Q01339 Mouse Beta-2-glycoprotein 1

Gene Symbol APOH
Gene Full Name apolipoprotein H (beta-2-glycoprotein I)
Background Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq, Jul 2008]
Function Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. [UniProt]
Cellular Localization Secreted. [UniProt]
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PTM N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. [UniProt]
Title Download Link
ARG82483 Mouse Apolipoprotein H ELISA Kit User manual download icon Download