ARG81951

Mouse Artemin ELISA Kit

Mouse Artemin ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81951-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81951-002 Standard 2 X 10 ng/vial 4°C
ARG81951-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81951-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81951-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81951-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81951-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81951-008 25X Wash buffer 20 ml 4°C
ARG81951-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81951-010 STOP solution 10 ml (Ready to use) 4°C
ARG81951-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81951 Mouse Artemin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Artemin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Artemin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.0%
Alternate Names ENOVIN; Neublastin; EVN; Artemin; Enovin; NBN

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11876 Mouse ARTN

Swiss-port # Q9Z0L2 Mouse Artemin

Gene Symbol ARTN
Gene Full Name artemin
Background The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Function Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. [UniProt]
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Artemin antibodies; Artemin ELISA Kits;
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Simplify the ELISA analysis by GainData
Title Download Link
ARG81951 Mouse Artemin ELISA Kit User manual download icon Download