ARG81275

Mouse BAFF ELISA Kit

Mouse BAFF ELISA Kit for ELISA and Mouse

Overview

Product Description ARG81275 Mouse BAFF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse BAFF in serum, plasma or cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name BAFF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 30 pg/ml
Sample Type Serum, plasma or cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Alternate Names BLYS; TALL1; Dendritic cell-derived TNF-like molecule; THANK; DTL; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF20; CD257; BLyS; Tumor necrosis factor ligand superfamily member 13B; ZTNF4; CD antigen CD257; B lymphocyte stimulator; TALL-1; BAFF; B-cell-activating factor

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24099 Mouse TNFSF13B

Swiss-port # Q9WU72 Mouse Tumor necrosis factor ligand superfamily member 13B

Gene Symbol Tnfsf13b
Gene Full Name tumor necrosis factor (ligand) superfamily, member 13b
Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
Function Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.

Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. [UniProt]
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PTM The soluble form derives from the membrane form by proteolytic processing.
Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
N-glycosylated.
Title Download Link
ARG81275 Mouse BAFF ELISA Kit User's manual download icon Download