ARG81275
Mouse BAFF ELISA Kit
Mouse BAFF ELISA Kit for ELISA and Mouse
Overview
| Product Description | ARG81275 Mouse BAFF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse BAFF in serum, plasma or cell culture supernatants. |
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| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | BAFF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, plasma or cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Alternate Names | BLYS; TALL1; Dendritic cell-derived TNF-like molecule; THANK; DTL; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF20; CD257; BLyS; Tumor necrosis factor ligand superfamily member 13B; ZTNF4; CD antigen CD257; B lymphocyte stimulator; TALL-1; BAFF; B-cell-activating factor |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9WU72 Mouse Tumor necrosis factor ligand superfamily member 13B |
|---|---|
| Gene Symbol | Tnfsf13b |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 13b |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
| Function | Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. [UniProt] |
| Highlight | Related products: BAFF antibodies; BAFF ELISA Kits; BAFF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated. |
| Title | Download Link |
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| ARG81275 Mouse BAFF ELISA Kit User's manual |
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