ARG81675

Mouse CD120a / TNFR1 ELISA Kit

Mouse CD120a / TNFR1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81675-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81675-002 Standard 2 X 10 ng/vial 4°C
ARG81675-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81675-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81675-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81675-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81675-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81675-008 25X Wash buffer 20 ml 4°C
ARG81675-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81675-010 STOP solution 10 ml (Ready to use) 4°C
ARG81675-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81675 Mouse CD120a / TNFR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD120a / TNFR1 in serum and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD120a / TNFR1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 6.2%
Alternate Names TNF-R; p60; TNFAR; CD antigen CD120a; TNFR55; TBP1; TNF-RI; TNFR1-d2; Tumor necrosis factor receptor superfamily member 1A; FPF; TNFR60; CD120a; TNFR1; p55; TNF-R55; TNF-R-I; MS5; TNFR-I; Tumor necrosis factor receptor 1; TBPI; Tumor necrosis factor receptor type I; TNF-R1; p55-R

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21937 Mouse TNFRSF1A

Swiss-port # P25118 Mouse Tumor necrosis factor receptor superfamily member 1A

Gene Symbol TNFRSF1A
Gene Full Name tumor necrosis factor receptor superfamily, member 1A
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq, Jul 2008]
Function Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. [UniProt]
Highlight Related products:
CD120a antibodies; CD120a ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The soluble form is produced from the membrane form by proteolytic processing. [UniProt]
Title Download Link
ARG81675 Mouse CD120a / TNFR1 ELISA Kit User's manual download icon Download