ARG81675
Mouse CD120a / TNFR1 ELISA Kit
Mouse CD120a / TNFR1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81675-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81675-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81675-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81675-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81675-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81675-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81675-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81675-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81675-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81675-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81675-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81675 Mouse CD120a / TNFR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD120a / TNFR1 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD120a / TNFR1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 6.2% |
| Alternate Names | TNF-R; p60; TNFAR; CD antigen CD120a; TNFR55; TBP1; TNF-RI; TNFR1-d2; Tumor necrosis factor receptor superfamily member 1A; FPF; TNFR60; CD120a; TNFR1; p55; TNF-R55; TNF-R-I; MS5; TNFR-I; Tumor necrosis factor receptor 1; TBPI; Tumor necrosis factor receptor type I; TNF-R1; p55-R |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25118 Mouse Tumor necrosis factor receptor superfamily member 1A |
|---|---|
| Gene Symbol | TNFRSF1A |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 1A |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq, Jul 2008] |
| Function | Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. [UniProt] |
| Highlight | Related products: CD120a antibodies; CD120a ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The soluble form is produced from the membrane form by proteolytic processing. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81675 Mouse CD120a / TNFR1 ELISA Kit User's manual |
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