ARG70314
Mouse CD120a / TNFR1 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Mouse CD120a / TNFR1 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Mouse CD120a / TNFR1 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | Binding, SDS-PAGE |
| Target Name | CD120a / TNFR1 (ECD) |
| Species | Mouse |
| A.A. Sequence | Met1 - Ala212 of Mouse CD120a / TNFR1 (NP_035739.2) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | TNF-R; p60; TNFAR; CD antigen CD120a; TNFR55; TBP1; TNF-RI; TNFR1-d2; Tumor necrosis factor receptor superfamily member 1A; FPF; TNFR60; CD120a; TNFR1; p55; TNF-R55; TNF-R-I; MS5; TNFR-I; Tumor necrosis factor receptor 1; TBPI; Tumor necrosis factor receptor type I; TNF-R1; p55-R |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse TNF-alpha at 500ng/ml (100 µl/well) can bind Recombinant Mouse TNFR1 with a linear range of 4-16 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25118 Mouse Tumor necrosis factor receptor superfamily member 1A |
|---|---|
| Gene Symbol | TNFRSF1A |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 1A |
| Background | This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016] |
| Function | Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Secreted. Note=A secreted form is produced through proteolytic processing. Isoform 4: Secreted. Note=Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | The soluble form is produced from the membrane form by proteolytic processing. [UniProt] |
