ARG81723

Mouse CD127 / IL7R ELISA Kit

Mouse CD127 / IL7R ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81723-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81723-002 Standard 2 X 20 ng/vial 4°C
ARG81723-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81723-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81723-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81723-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81723-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81723-008 25X Wash buffer 20 ml 4°C
ARG81723-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81723-010 STOP solution 10 ml (Ready to use) 4°C
ARG81723-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81723 Mouse CD127 / IL7R ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD127 / IL7R in serum and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD127 / IL7R
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.7%
Alternate Names ILRA; CD127; IL-7R subunit alpha; IL-7R-alpha; IL7RA; CD antigen CD127; Interleukin-7 receptor subunit alpha; CDW127; CDw127; IL-7 receptor subunit alpha; IL-7RA

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16197 Mouse IL7R

Swiss-port # P16872 Mouse Interleukin-7 receptor subunit alpha

Gene Symbol IL7R
Gene Full Name interleukin 7 receptor
Background The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2014]
Function Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). [UniProt]
Highlight Related products:
CD127 antibodies; CD127 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. [UniProt]
Title Download Link
ARG81723 Mouse CD127 / IL7R ELISA Kit User's manual download icon Download