ARG81711

Mouse CD14 ELISA Kit

Mouse CD14 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81711-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81711-002 Standard 2 X 10 ng/vial 4°C
ARG81711-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81711-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81711-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81711-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81711-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81711-008 25X Wash buffer 20 ml 4°C
ARG81711-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81711-010 STOP solution 10 ml (Ready to use) 4°C
ARG81711-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81711 Mouse CD14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD14 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD14
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%; Inter-Assay CV: 6.0%
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12475 Mouse CD14

Swiss-port # P10810 Mouse Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Highlight Related products:
CD14 antibodies; CD14 ELISA Kits; CD14 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt]
Title Download Link
ARG81711 Mouse CD14 ELISA Kit User's manual download icon Download