ARG81776

Mouse CD25 ELISA Kit

Mouse CD25 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81776-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81776-002 Standard 2 X 10 ng/vial 4°C
ARG81776-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81776-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81776-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81776-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81776-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81776-008 25X Wash buffer 20 ml 4°C
ARG81776-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81776-010 STOP solution 10 ml (Ready to use) 4°C
ARG81776-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81776 Mouse CD25 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD25 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD25
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 39 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 78 - 5000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.0%
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16184 Mouse IL2RA

Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
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Title Download Link
ARG81776 Mouse CD25 ELISA Kit User manual download icon Download