ARG82634
Mouse CD253 / TRAIL ELISA Kit
Mouse CD253 / TRAIL ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82634-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82634-002 | Standard (Lyophilized) | 2 X 10 ng/vial | 4°C |
| ARG82634-003 | Standard/Sample diluent | 30 ml | 4°C |
| ARG82634-004 | Antibody conjugate concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG82634-005 | Antibody diluent buffer | 12 ml | 4°C |
| ARG82634-006 | HRP-Streptavidin concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG82634-007 | HRP-Streptavidin diluent buffer | 12 ml | 4°C |
| ARG82634-008 | Wash Buffer (20X) | 30 ml | 4°C |
| ARG82634-009 | TMB substrate | 12 ml | 4°C (Protect from light) |
| ARG82634-010 | STOP solution | 12 ml | 4°C |
| ARG82634-011 | Plate sealer | 3 strips | Room temperature |
Overview
| Product Description | ARG82634 Mouse CD253 / TRAIL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD253 / TRAIL in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | No detectable cross-reactivity with other relevant proteins. |
| Target Name | CD253 / TRAIL |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Alternate Names | TL2; CD253; Protein TRAIL; TNF-related apoptosis-inducing ligand; TRAIL; CD antigen CD253; Apo-2 ligand; Apo-2L; APO2L; Tumor necrosis factor ligand superfamily member 10 |
Application Instructions
| Assay Time | ~ 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P50592 Mouse Tumor necrosis factor ligand superfamily member 10 |
|---|---|
| Gene Symbol | TNFSF10 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 10 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Function | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. [UniProt] |
| Cellular Localization | Membrane; Single-pass type II membrane protein. [UniProt] |
| Highlight | Related products: CD253 antibodies; CD253 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82634 Mouse CD253 / TRAIL ELISA Kit User manual |
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