ARG70370

Mouse CD254 / RANKL recombinant protein (Active) (His-tagged, C-ter)

Mouse CD254 / RANKL recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE and Mouse

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Mouse CD254 / RANKL recombinant protein
Tested Reactivity Ms
Tested Application SDS-PAGE
Target Name CD254 / RANKL
Species Mouse
A.A. Sequence MPAMM EGSWL DVAQR GKPEA QPFAH LTINA ASIPS GSHKV TLSSW YHDRG WAKIS NMTLS NGKLR VNQDG FYYLY ANICF RHHET SGSVP TDYLQ LMVYV VKTSI KIPSS HNLMK GGSTK NWSGN SEFHF YSINV GGFFK LRAGE EISIQ VSNPS LLDPD QDATY FGAFK VQDID with polyhistidine tag at the C-terminus.
Expression System E. coli
Protein Full Name tumor necrosis factor ligand superfamily member 11
Activity Active
Activity Note Determined by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is <2 ng/mL.
Alternate Names TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21943 Mouse TNFSF11

Swiss-port # O35235 Mouse Tumor necrosis factor ligand superfamily member 11

Gene Symbol TNFSF11
Gene Full Name tumor necrosis factor (ligand) superfamily, member 11
Background This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt]
PTM The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.