ARG70229
Mouse CD274 / PD-L1 recombinant protein (His-tagged, C-ter)
Mouse CD274 / PD-L1 recombinant protein (His-tagged, C-ter) for SDS-PAGE and Mouse
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Mouse CD274 / PD-L1 recombinant protein. |
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| Tested Reactivity | Ms |
| Tested Application | SDS-PAGE |
| Target Name | CD274 / PD-L1 |
| Species | Mouse |
| A.A. Sequence | Met1 - Thr238 of Mouse CD274 / PD-L1 (NP_068693) with polyhistidine tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-L1 at 10 µg/ml (100 µl/well) can bind Recombinant Mouse PD-1 with a linear range of 1.5 - 5 µg/ml. |
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Properties
| Form | Powder |
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| Purification Note | 0.22 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CD274 |
| Gene Full Name | CD274 molecule |
| Background | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Endomembrane system; Single-pass type I membrane protein. [UniProt] |
