ARG81342
Mouse CD279 / PD-1 ELISA Kit
Mouse CD279 / PD-1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81342-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81342-002 | Standard (Lyophilized) | 2 X 1.6 ng/vial | 4°C |
| ARG81342-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG81342-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG81342-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG81342-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG81342-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG81342-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG81342-009 | TMB substrate | 12 ml | 4°C (Protect from light) |
| ARG81342-010 | STOP solution | 12 ml | 4°C |
| ARG81342-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81342 Mouse CD279 / PD-1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD279 / PD-1 in serum, plasma and cell culture supernatant. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | CD279 / PD-1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatant. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1 |
Application Instructions
| Assay Time | ~ 3.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDCD1 |
| Gene Full Name | programmed cell death 1 |
| Background | CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008] |
| Function | CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt] |
| Highlight | Related products: PD-1 antibodies; PD-1 ELISA Kits; PD-1 Duos / Panels; Related news: New PD-1 ELISA Kit, excellent for preclinical studies or pharmatheutical development The best solution for PD-1/PD-L1 research Examining CTL/NK-mediated cytotoxicity by ELISA New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG81342 Mouse CD279 / PD-1 ELISA Kit User's manual |
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